4 x 400 Fresh-Soph
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| PL | TEAM | ATHLETES | TIME | TIME | TIME | TIME |
|---|---|---|---|---|---|---|
| 1 | Mainland | Marin Hawryluk, Taliyah Nixon, Autumn Demarest, Saraya Hinson | 4:54.00 | 5:43.98 | 3:52.26 | 3:52.26 |
| 2 | DeLand | Sydney Neira, Amy Nguyen, Sydney Bordeaux, Lilah Kersey | 5:04.19 | 5:34.61 | 3:54.23 | 3:57.27 |
| 3 | University (OC) | Lillie Pickens, Alea Smith, Shelsy Burgos Rosado, Sarahi Arroyo Canales | 5:33.84 | 7:00.64 | 5:37.18 | 6:53.97 |
| 4 | Lake Weir | 5:44.64 | 4:11.59 | 4:35.72 | 4:21.93 | |
| Father Lopez | Bianca Campos, Adelae Fulton, Marissa Stanberry, Frances Ginnochetti | DNS | DNS | DNS | DNS | |
| Olympia | Sandy Scott, Maddie Heald, Arlinda Roundtree, Korey Ardon | DNS | DNS | DNS | DNS |